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sa can be disabled from the security section. right-click on the account, select properties, and tick the login disabled option (i use 2005, so not exactly sure how it looks).
That will stop its access entirely, but if you want to stop its access to a specific database. Not really. the sa account is the full admin account on the server, its in the sysadmin role, and so has access to everything. you could remove it from that, but then what if you really need the sa accounts level of access?
"Im Nerdy in the extreme and whiter than sour cream"